Hello, I have 2 different assignments that are due on Sunday 12.02.18: MILESTONE 1 and MILESTONE 2.Please note that these are TWO different assignments that coincide together, therefore TWO different 1 page papers are needed.I have attached the rubric for each milestone below.Thanks!
Hello, I have 2 different assignments that are due on Sunday 12.02.18: MILESTONE 1 and MILESTONE 2.Please note that these are TWO different assignments that coincide together, therefore TWO different
Salmonella in the Caribbean Modifed from “Stehr -Green, J., Caribbean Epidemiology Centre: Pan American Health Organization, World Health Organization, & Centers for Disease Control and Prevention. (2016, April 14). Epidemiologic Case Studies (Rep.). Retrieved from https://www.cdc.gov/epicasestudies/classroom_salmonella.html” Original investigators: Lisa Indar -Harrinauth, 1, 2 Nicholas Daniels, 3 Parimi Prabbakar, 1 Clive Brown, 1 Gail Baccus -Taylor, 2 Edward Commissiong, 2 H. Reid, 4 and James Hosp edales 1 1Caribbean Epidemiology Centre, Pan American Health Organization/World Health Organization 2Food Technology Unit, Department of Chemical Engineering, University of the West Indies 3Foodborne and Diarrheal Diseases Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention 4Trinidad Public Health Laboratory, Trinidad Case study and instructor’s guide created by: Jeanette K. Stehr -Green, MD Reviewed by: Frederick J. Angulo, DVM, PhD, Stephanie M. DeLong, MPH, Lisa Indar – Harrinauth, PhD, MSc, James Hospedales, MBBS, MSc, MFPHM, Robert Tauxe, MD, MPH, James Flint, MPH, Roderick C. Jones, MPH, Eleni Galanis, MD, MPH NOTE: This case study is based on real -life investigations undertaken in Trinidad and Tobago in 1998 -1999 and published in Clinical Infectious Diseases and the West Indian Medical Journal . (See Appendix for abstracts.) Some aspects of these investigations (and the circumstances leading up to them) have been altered to assist in meeting the desired teaching objectives and some details have been fabricated to provide continuity to the storyline. Target audience: public health practitioners with knowledge of basic epidemiologic concepts, especia lly non -epidemiologists (e.g., laboratorians, environmental health specialists, sanitarians, public health nurses, veterinarians, MPH students) Training materials funded by: the Centers for Disease Control and Prevention (National Center for Infectious Di seases, Food Safety Initiative, Public Health Practice Program Office, and Epidemiology Program Office/Division of International Health) August 2004 U.S. DEPARTMENT OF HEALTH AND HUMAN SERVICES Public Health Service Centers for Disease Control and Prevention Atlanta, Georgia 30333 Salmonella in the Caribbean Part I – Background on Salmonella Salmonellosis is a gastrointestinal illness caused by bacteria from the genus Salmonella . The illness is characterized by the sudden onset of headache, abdominal pain, diarrhea (which may be bloody), nausea, and sometimes vomiting. Fever is almost always p resent. The illness typically lasts for 5 -7 days and usually does not require treatment unless the patient becomes severely dehydrated or the infection spreads from the intestines. In the immunocompromised host or an overwhelming infection in a normal host , Salmonella may spread to the blood stream and other body sites, and can cause death unless treated promptly with antibiotics. Salmonella live in the intestinal tracts of humans and other animals, including mammals, birds, and reptiles. Salmonella are usually transmitted to humans by eating foods contaminated with animal feces. Implicated foods are typically those of animal origin, such as beef, poultry, milk, or eggs, but all foods, including vegetables, may become contaminated. The incubation period f or salmonellosis is usually 12 – 36 hours, but can be as long as a week. Figure 1. Salmonella surface antigens The genus Salmonella consists of only two species: S. enterica and S. bongori . The latter species, however, is very rare. Members of the species Salmonella enterica can be divided into smaller groups (i.e., serotypes) based on two structures on the cell surface: the O antigen and the H antigen. The O antigen is a carbohydrate antigen in the lipopoly saccharide of the bacterium’s outer cell membrane. The H antigen is a protein antigen in the bacterium’s flagella. (Figure 1) O antigens and H antigens are detected using antisera that react with a single antigen or group of related antigens. All Salmonel la serotypes can be designated using a formula based on the O and H antigens they express. Many serotypes are also given a name (e.g., Salmonella Typhimurium, Salmonella Agona, Salmonella Muenchen). (NOTE: The serotype name is capitalized and not italicize d.) Although extensive serotyping of surface antigens can be used for identification of a Salmonella isolate, the reagents are costly, the process is time -consuming, and the results are not likely to affect treatment of the individual patient. As a result , in many countries clinical laboratories perform only a few O antigen reactions that allow them to group an isolate into broader, less specific categories called serogroups. The isolate is then forwarded to a state or national reference laboratory for com plete serotyping. There are over 2,500 recognized Salmonella serotypes. In 1995, Salmonella Enteritidis, Typhimurium, and Typhi accounted for over three -quarters of the isolates reported in a global survey. Part II – Surveillance of Sal monella in the Caribbean As early as the mid -1980s, Salmonella became a pathogen of public health concern in the Caribbean (Figure 2) when it caused an increasing number of cases and outbreaks of diarrhea involving local and tourist populations. The communicable disease surveillance system in place at the time, howeve r, did not support the timely detection of these outbreaks or the investigation of risk factors associated with infection. As a result, the incidence of Salmonella continued to grow. Figure 2. Countries of the Caribbean and surrounding land masses. The communicable disease surveillance system in the Caribbean was based on notifiable disease reports from physicians and other health care providers in the community (i.e., clinician -based reporting). Surveillance of most communicable di seases included both laboratory -confirmed cases and cases diagnosed based on clinician suspicion. The laboratory did not report cases of communicable disease to the surveillance system or submit isolates for confirmation or further testing (e.g., serotypin g). To report a communicable disease in the Caribbean, the health care provider completed a disease report card (Figure 3) and mailed it to the local health department within 7 days of diagnosis of the patient. Figure 3. Communicable Disease Case Repor t Card CARIBBEAN EPIDEMIOLOGY CENTRE Clinician -based Reporting COMMUNICABLE DISEASE CASE REPORT CARD Case identification Last name, First name, Middle initial: Address: City/Country: Disease information Diagnosis: Lab -confirmed: _ Yes _ No Date of onset: Case information Sex: _ Male _ Female Age: Current status: _ Alive _ Dead Attending physician Name: Address: Telephone number: Person reporting case (if not attending physician) Name: Telephone number: A clerk at the local health department reviewed the report cards for completeness (requesting additional information from the health care provider, where needed), batched the reports, and mailed them to the country’s Ministry of Health where they were sorted by disease. The Ministry of Health forwarded the reports to the Caribbean Epidemiology Centre (CAREC). An epidemiologist from CAREC reviewed and summarized the reports from the individual countries. If necessary, the epidemiologist contacted the Ministry of Health if an unusual disease pattern was noted. CAREC distributed weekly, quarterly, and annual communicable disease reports for the region and each country to all Ministries of Health. In addition, CAREC reported occurrences of selected diseases (e.g. , cholera, plague, yellow fever) to the Pan American Health Organization/World Health Organization as required by International Health Regulations. In an evaluation of the Caribbean communicable disease surveillance system, it was determined that less than 40% of notifiable disease cases were actually reported by health care providers. The average reporting delay (i.e., from diagnosis to receipt of the report by CAREC) was 56 days. After extensive consultation with the Ministries of Health from the ind ividual Caribbean countries, health care providers, professional medical organizations, and clinical laboratories, CAREC proposed a modification of communicable disease reporting in the region. In addition to health care providers (i.e., clinician -based s urveillance), clinical laboratories were enlisted to report the detection of notifiable diseases that were laboratory confirmed (i.e., laboratory -based surveillance). Clinical laboratories were also asked to forward all isolates of Salmonella to the nation al reference laboratory in Trinidad (i.e., the Public Health Laboratory). Staff at the Public Health Laboratory, who had specialized training in Salmonella serotyping and access to specialized reagents, were to perform serotyping and antimicrobial suscepti bility testing on forwarded Salmonella isolates. To further improve the timeliness of reporting, health care providers and clinical laboratories were to submit reports directly to newly designated surveillance officers in each country’s Ministry of Health . Diseases that potentially could be spread through food or water or readily from person -to-person were to be reported within 24 hours of diagnosis. The remainder were to be reported within 3 days of diagnosis. Health care providers and clinical laboratori es were encouraged to submit reports by telephone or FAX. Initial acceptance and implementation of the new communicable disease reporting procedures were slow. Member countries had limited public health resources to initiate the changes and there was resi stance among health care providers and clinical laboratories. Staff from CAREC visited member countries and, with the assistance of staff from the local Ministry of Health, provided training to both health care providers and staff from clinical laboratori es. Training focused on the mechanics of reporting and how surveillance data would be used to monitor disease trends, detect outbreaks, and initiate controls measures. Many of the presentations were made at professional meetings, allowing for an open discu ssion of the reporting procedures and surveillance in general. CAREC staff toured the larger clinical laboratories in the various countries and identified problems associated with testing, reporting, and the forwarding of Salmonella isolates to the nation al Public Health Laboratory in Trinidad. A resource person was identified at the Public Health Laboratory to provide ongoing support to all clinical laboratories. A close working relationship developed between the Public Health Laboratory in Trinidad and CAREC. Laboratory staff forwarded laboratory results to epidemiologists at CAREC on a weekly basis and notified them by phone if an unusual case was noted or an increase in the isolation rate of a particular disease occurred. CAREC staff summarized commun icable disease surveillance results (including serotype and antimicrobial susceptibility test results) and distributed a weekly summary to the Ministries of Health and monthly updates to health care providers and clinical laboratories. They worked closely with staff from the respective Ministries of Health if an unusual disease pattern was noted or some reporting problem became evident. Part III – Descriptive Epidemiology of Salmonella in Trinidad Due to the close proximity of both CAREC and the national Public Health Laboratory, Trinidad and Tobago moved most quickly on the implementation of the new reporting procedures. As a result, several large outbreaks of salmonellosis were detected allowing local public health practitioners to initi ate investigations and implement appropriate control measures. However, salmonellosis continued to occur at a high rate in the country. In 1998, CAREC summarized the following data for laboratory -confirmed cases of salmonellosis reported in Trinidad and T obago. Table 1. Laboratory isolates of Salmonella by serotype and year of diagnosis, Trinidad and Tobago, 1988 -1997. Serotype Year of Diagnosis 88 89 90 91 92 93 94 95 96 97 Enteritidis 0 0 0 0 10 12 28 57 117 83 Typhimurium 14 16 19 27 94 55 47 23 21 15 Other 37 28 37 58 31 47 54 59 67 41 TOTAL 51 44 56 85 135 114 129 139 205 139 Due to the increase in S. Enteritidis in Trinidad and Tobago, CAREC focused their analyses on this serotype. The following data are for S. Enteritidis only. From 1995 -97, 257 laboratory -confirmed cases of S. Enteritidis infection were reported in Trinidad and Tobago. Approximately, 86 cases were reported each year for an annual incidence of 6.8 per 100,000 population. In general, the geographic distribution of patients with S. Enteritidis infection reflected population distributions on the two islands. The largest numbers of cases were reported from the most populous counties of St. George and Victoria. An average of 4 people died each year from salmonella infe ction. A similar proportion of S. Enteritidis infections occurred among males (48%) and females (52%). However, the distribution of cases varied by age group (Figure 4) and month of diagnosis (Figure 5). Figure 4. Laboratory -confirmed cases of Salmonella Enteritidis (per 100,000 population) by age group and year of diagnosis, Trinidad and Tobago, 1995 -1997. Figure 5. Laboratory -confirmed cases of Salmonella Enteritidis by month and year of diagnosis, Trinidad and Tobago, 1995 -1997. Part IV – Case -Control Study of S. Enteritidis in Trinidad and Tobago To explore risk factors for S. Enteritidis infection in Trinidad and Tobago, a matched case -control study was undertaken from March 1998 – May 1999. A case -control study design was u sed because the cases did not arise from a well -defined group of people and were distributed across the entire country. Cases were patients with laboratory -confirmed S. Enteritidis infection who were reported through the communicable disease surveillance system. Cases were enrolled prospectively, shortly after diagnosis. Controls were persons with no diarrheal illness in the previous 4 weeks who lived in the same neighborhood as cases and were similar in age. Investigators attempted to enroll two controls for each case. Using a standardized questionnaire, investigators collected information from cases about foods and beverages consumed, recent travel, and food handling practices in the 3 days before they became ill. Controls were asked about these exposure s during the same 3 -day period as the matched case. The questionnaire was administered to both cases and controls by one of the investigators in face -to-face interviews. Forty -five patients and 92 controls were enrolled in the case -control study. The investigators analyzed the results of the case -control study. In the Trinidad and Tobago case -control study, cases and controls were similar to each other in terms of age, sex, ethnic distribution, and place of residence. Exposure to potential sources of Salmonella , however, differed between cases and controls (Table 2). Table 2. Potential sources of exposure to Salmonella , Trinidad and Tobago Case -Control Study, March 1998 – May 1999. Exposure * Matched Odds Ratio p-value Ate chicken 0.5 0.4 Ate shell eggs 8.8 <0.001 Ate dishes that contained raw or undercooked eggs 18.9 0.001 Ate ground beef 1.3 0.6 Ingested powdered milk 1.5 0.2 Exposed to live chickens 1.3 0.4 Bought refrigerated eggs 0.1 <0.001 Refrigerated eggs at home 0.03 <0.001 *in the 3 days before onset of illness in the associated case The specific raw egg -containing foods that were implicated by the case patients’ food histories included homemade eggnog, cake batter, homemade ice cream, punch a crème (i.e., a drink similar to eggnog), and stout and eggs. The implicated food items correlated with the predominance of cases in December and January as many of these foods are consumed more frequently in the holiday season. Samples of the implicated foods were collected from patients, from the places where patients had originally purchased the foods, or both and were cultured for Salmonella . S. Enteritidis isolates from patients and food were phage -typed at the U.S. Centers for Disease Control and Prevention (CDC) in Atlanta, Georgia. S. Enteritidis samples from 34 patients were selected for phage typing. Of these, 30 (88%) were found to be phage type 4 and 4 (12%) were found to be phage type 1. S. Enteritidis was isolated from 15 (45%) of the 33 food samples implicated by the patients’ fo od histories. Nine of the 15 food isolates were phage typed; all nine were phage type 4. Part V – Study of Eggs in Trinidad To further investigate the role that eggs may play as a source of Salmonella infections in Trinidad, a microbiologic survey of shell eggs was undertaken. Ten egg -producing farms across the country were selected, nine of which were the largest and most popular commercial table egg farms in Trinidad. Their total production accounted for approximately 75% of the country’s egg supply. Twenty -five freshly laid eggs were collected from each farm on three different occasions for a total of 750 eggs. Each set of 25 eggs was cultured for Salmonella in pooled batches. Eggshells were cultured separately from egg contents. The eggshell sampl es were prepared by swabbing the shell surface of each of the 25 eggs with cotton wool tips moistened with lactose pre -enrichment broth. The eggs were not washed prior to swabbing. The eggs were then sanitized using U.S. Food and Drug Administration eggsh ell disinfection procedures: each egg was cleaned with a stiff wire brush, hand washed under running water with antibacterial soap, and patted dry with a paper towel. The eggs were then placed in a wire basket and immersed in 70% alcohol for 30 minutes fol lowed by distilled water for 10 minutes. The eggs were then allowed to air dry. The contents were removed aseptically, pooled together, and homogenized in a blender. Salmonella was detected more often in shell cultures (4.6% of samples) than in content cu ltures (1.2% of samples). S. Typhimurium was the most prevalent serotype found on the eggshells and S. Enteritidis was the most prevalent serotype isolated from the egg contents (Table 3). Table 3. Salmonella serotypes isolated from the 750 pooled eggshel ls and egg contents from 10 egg -producing farms, Trinidad, 1998 -1999. Salmonella serotype isolated Percent positive for serotype * Pooled eggshells Pooled egg contents S. Typhimurium 3.06 0.4 S. Enteritidis 0.67 0.8 S. Ohio 0.27 - S. Cerro 0.27 - S. Infantis 0.27 - S. Heidelberg 0.13 - Total 4.6 1.2 *Because Salmonella isolates are generally present in very low numbers in eggs, it was assumed that each isolate came from one positive egg and the percentage was based on a denominator of 750. The isolation rates of Salmonella on shell surfaces and in egg contents varied among the 10 egg - producing farms. At least one serotype of Salmonella was isolated from eggshells at all 10 of the farms. Salmonella was isolated from egg contents at only thre e of the farms. An environmental health assessment was undertaken at each of the farms by a food safety officer from the Trinidad and Tobago Ministry of Agriculture to identify factors that could have contributed to the contamination of eggshells and cont ents with Salmonella . The food safety officer inspected the farms and collected information about the system of chicken rearing, quality control measures, feed and litter type, egg cleanliness, and other management practices. At four of the farms, the environment and immediate surroundings were generally clean with dry litter surfaces and clean drinking water, poultry houses, nesting boxes, and equipment. Proper egg -handling techniques and good farm practices were also employed. The eggs collected from these farms appeared clean with little or no fecal matter on their surfaces. In contrast, the surroundings of the other six egg -producing farms generally appeared unsanitary: litter surfaces were wet on most occasions. Egg belts, poultry houses, and nesti ng boxes were dirty and there were rodents and flies. These farms were also characterized by odor build -up, such as ammonia, and the eggs collected from them frequently had feces and sometimes blood on the shells. In general, these farms had higher Salmone lla isolation rates from pooled eggshells and egg contents than the other farms. None of the 10 farms had routine microbial monitoring of their flocks or eggs. Part VI - Prevention and Control Following release of the results from the S. Enteritidis case -control study, the microbiologic survey of shell eggs, and environmental health assessments of egg -producing farms, the Trinidad and Tobago Ministries of Health and of Agriculture initiated a farm -to-table approach to Salmonella prevention and contro l strategies. These strategies combined public health education of consumers, food service establishments, and food workers (on the risks associated with eating raw and undercooked eggs and using unrefrigerated eggs) and strategies for reduction of Salmone lla infections among egg -laying flocks and breeder flocks. Regional workshops were held in November 2002 for egg producers on production and food safety. “Good Agricultural Practices” for hatchery sanitation and egg production were developed from the proc eedings. Drafts were widely distributed for review and comment. Final copies were distributed to all egg -producing farms under the coordination of the Inter -American Institute for Cooperation on Agriculture. The Ministry of Agriculture, responsible for th e regulation of food safety in Trinidad and Tobago, made staff available on an ongoing basis to answer questions from producers on the “Good Agricultural Practices” and help them to explore and solve problems. Through public and private partnerships and networking, Ministry of Agriculture officials developed a protocol to identify and remove infected flocks from the egg supply and increase quality assurance and sanitation measures at egg -producing farms. The procedures included the following steps: Both eggs and chickens from commercial egg -producing farms will be tested for Salmonella on a quarterly basis. Any flocks that test positive for Salmonella on routine exam will be re -tested. If a second sample is positive, traceback investigations will be under taken to identify breeder flocks. Infected breeder flocks (those that produced the egg -laying chickens) will be slaughtered. Eggs from infected egg -laying chickens will be pasteurized instead of being sold as shell eggs. Non -infected flocks from farms at w hich infected flocks have been detected will be tested more frequently (i.e., every 4 weeks). The Ministry of Agriculture implemented the above procedures in Trinidad and Tobago in 2003. In 2004, the Ministry of Agriculture took additional efforts to pre vent the occurrence of salmonella by implementing strategies to reduce the prevalence of the disease and stop the transmission across the population. Specifically, the Ministry of Health implemented strategies to increase the timeliness of reporting salmon ella cases. With the initiative, the position of “Surveillance Officer” was created, and surveillance officers were hired for each country’s Ministry of Health. The new protocol required health care providers and clinical laboratories to submit reports of disease detection directly to the surveillance officer, ensuring a specific individual is in charge of tracking the occurrence and prevalence of the diseases. In addition, diseases that potentially could be spread through food or water, or readily from per son -to-person, were to be reported within 24 hours of diagnosis. The remainder were to be reported within 3 days of diagnosis. Health care providers and clinical laboratories were encouraged to submit reports by telephone or FAX. In 2006, the Ministry of Agriculture further expanded their prevention strategy by offering treatment for the disease. Although antibiotics are not usually required for a salmonella outbreak as most people recover on their own within a week, there are extreme cases in which antibi otics may be necessary. Previously, antibiotics were not designated for salmonella outbreaks, but with the new initiative, those with severe cases of salmonella are able to access antibiotics for their treatment. In addition, the Ministry of Agriculture ha s strengthened their communication to the community around common treatment options for the disease, and strategies for alleviating common symptoms. Specifically, there has been a campaign educating parents and children on the importance of hydration when affected by salmonella, and the dangers of dehydration, which is one of the most dangerous aspect of salmonella. Epilogue Serotyping of Salmonella , as used in the investigation of Salmonella Enteritidis in the Caribbean, is a common subtyping procedure used throughout the world. In a 1997 survey of World Health Organization (WHO) member states, 69 of the responding 104 countries conducted Salmonella serotyping as part of public health surveillance for sa lmonellosis. Serotyping was performed in all six WHO regions; however, surveillance was limited in time or scope for some countries. Access to serotyping reagents varied by country and some countries reported only serogroup results. WHO Global Salm -Surv , an international, foodborne disease surveillance network, was created by WHO in partnership with the Danish Institute for Food and Veterinary Research, the Centers for Disease Control and Prevention, Institut Pasteur International Network, Health Canada, and the Animal Sciences Group (ID -Lelystad) in the Netherlands. The goal of WHO Global Salm -Surv is to reduce foodborne diseases through enhancement of laboratory -based surveillance (including serotyping and antimicrobial resistance testing) and outbreak detection and response techniques. Components of the network that help promote this goal include international training courses, an external quality assurance system, and country and region -specific projects. The network also offers a moderated list serv, web -based annual Salmonella summary data from member institutions, and a website, and provides services such as reference testing and identification of reliable sources of antisera for countries. As of November 2003, WHO Global Salm -Surv had members from 138 countries including the Bahamas, Barbados, Belize, Dominican Republic, Jamaica, Saint Lucia, Suriname, and Trinidad and Tobago in the Caribbean. Participation in WHO Global Salm -Surv has provided critical information to investigate outbreaks such as th e one described in this case study and has led to local interventions that have reduced the human health burden of Salmonella and other foodborne diseases globally. Figure 6. WHO Global Salm -Surv Country Membership References Herikstad H, Motarjemi Y, Tauxe RV. Salmonella surveillance: a global survey of public health serotyping. Epidemiol. Infect 2002;129:1 -8. Indar -Harrinauth L, Daniels N, Prabhakar P, Brown C, Baccus -Taylor G, Comissiong E, Hospedales J. Emergence of Salmon ella enteritidis phage type 4 in the Caribbean: Case -control study in Trinidad and Tobago, West Indies. Clinical Infectious Diseases 2001;32:890 -6. (See Appendix for abstract.) Indar L, Baccus -Taylor G, Commissiong E, Prabhakar P, Reid H. Salmonellosis in Trinidad: evidence for transovarian transmission of Salmonella in farm eggs. West Indian Med J 1998;47:50 -3. (See Appendix for abstract.) Orrett FA and Shurland SM. Susceptibility patterns and serotypes of non -typhoidal Salmonella inTrinidad. Saudi Med J 2001;22:852 -5. Appendix: Abstracts from Original Investigations Indar -Harrinauth L, Daniels N, Prabhakar P, Brown C, Baccus -Taylor G, Comissiong E, Hospedales J. Emergence of Salmonella enteritidis phage type 4 in the Caribbean: Case - contr ol study in Trinidad and Tobago, West Indies. Clin Infect Dis 2001;32(6):890 -6. A prospective case -control study involving 46 case patients and 92 age - and neighborhood -matched control subjects was conducted in Trinidad and Tobago (T& T) between March 1998 and May 1999 to determine the etiology, sources, and risk factors for Salmonella enteritidis (SE) infection. SE infection in T&T was found to be associated with the consumption of shell eggs, and in particular raw or undercooked eggs. SE isolates from 30 (88%) of 34 patients and from 9 implicated egg or egg -containing food samples were phage type 4. Homemade eggnog and ice cream, cake batter, and egg -containing beverages were the main raw egg -containing foods, reflecting the cultural practices of the people of T&T. Public health education on the risks of eating raw or undercooked e ggs, thorough cooking of all egg dishes, and refrigeration of shell eggs and egg dishes; studies tracing infected eggs to their sources; and testing of flocks of layer chickens for SE are needed to reduce the incidence of this infection. Indar L, Baccu s-Taylor G, Commissiong E, Prabhakar P, Reid H. Salmonellosis in Trinidad: Evidence for transovarian transmission of Salmonella in farm eggs. West Indian Med J 1998;47(2):50 -3. The aim of this study was to determine whether the contents of farm eggs in Tr inidad are contaminated with Salmonella and if transovarian transmission occurs. 750 fresh eggs from 10 farms supplying 75% of the country's eggs were cultured for Salmonella . Salmonella was found on the egg shells' surfaces from all farms, and in the egg contents from three farms. Isolates were obtained from the cultures of the contents and shells of nine (1.2%) and 35 (4.66%) eggs, respectively (p < 0.005). Serotypes found in the contents were S. enteritidis (0.8%; deduced to be contaminated by transovari an transmission) and S. typhimurium (0.4%); those isolated from the shells (contaminated by faecal transmission) were S. typhimurium (3.06%), S. enteritidis (0.67%), S. ohio (0.27%), S. cerro (0.27%), S. infantis (0.27%) and S. heidelberg (0.13%). This st udy provides the first evidence for Salmonella and, more importantly, S. enteritidis , in eggs in Trinidad. This is of major public health significance because S. enteritidis infected eggs appear normal and the organism is difficult to detect and control. T he consumption of these eggs may increase the risk of Salmonella infection. Food safety practices, particularly the thorough cooking (> or = 70 degrees C) of all egg dishes and the refrigeration (< 10 degrees C) of shell eggs and egg dishes, are recommende d. Hello, I have 2 different assignments that are due on Sunday 12.02.18: MILESTONE 1 and MILESTONE 2.Please note that these are TWO different assignments that coincide together, therefore TWO different IHP 330 Milestone One Guidelines and Rubric Overview: For your final project, you will complete an epidemiological research study report based on the provided research study, “Salmonella in the Caribbean .” Throughout the course, you will compl ete milestone assignments that are drafts of specific sections of the final project. Because these milestone assignments are drafts of the final project, you will notice that some critical elements in the rubric are identical to those in the Final Project Rubric. Tips for tackling each critical element are in italics throughout the document. In your first milestone, you will review the research study and analyze the health issue at its core. Use the research you co nducted in your Module One journal assignm ent to help you analyze the health issue. Prompt: To begin, review the final project research study, “Salmonella in the Caribbean.” Then, address the following critical elements in your short paper: II. Health Issue : In this section of your final project, yo u will analyze the health issue in focus in the research study. A. Describe the risk factors associated with the public health issue. Support your description with evidence from the research study and your independent research. B. Describe the mode of transmis sion of the public health issue. Support your description with evidence from the research study and your independent research. C. Determine the incidence and prevalence of the health issue using the data from the research study to perform the appropriate cal culations. Tip: Use the data in Table 1 of the final project research study to calculate the incidence of the Total of Serotypes. Also, use t he incidence to help you determine the prevalence of the disease. D. Determine the mortality rate of the health issue using the data from the research study to perform the appropriate calculations. E. Interpret the odds ratio to determine the strength of association between the potential sources of exposure and the disease. Tip: Use the data in Table 2 of the final project research study to calculate the odds ratio. Rubric Guidelines for Submission: Your paper should be a 1 - to 2 -page Microsoft Word document with double spacing, 12 -point Times New Roman font, one -inch margins, and APA formatting and citations. Critical Elements Proficient (100%) Needs Improvement (70%) Not Evident (0%) Value Health Issue: Risk Describes the risk factors Describes the risk factors Does not describe the risk 18 Factors associated with the public associated with the public factors associ ated with the health issue, supporting health issue, supporting public health issue description with evidence from description with evidence from the research study and from the research study and from independent research independent researc h, but response has gaps in detail, clarity, accuracy, or support Health Issue: Mode Describes the mode of Describes the mode of Does not describe the mode of 18 of Transmission transmission of the public transmission of the public transmissi on of the public health issue, supporting health issue, supporting health issue description with evidence from description with evidence from the research study and from the research study and from independent research independent research, b ut response has gaps in detail, clarity, accuracy, or support Health Issue: Determines the incidence and Determines the incidence and Does not determine the 18 Incidence and prevalence of the health issue prevalence of the health issue incide nce and prevalence of the Prevalence using the data from the using the data from the health issue using the data research study and performing research study and performing from the research study the appropriate calculations calculations, but calc ulations are inappropriate, or determination has gaps in detail, clarity, or logic Health Issue: Determines the mortality rate Determines the mortality rate Does not determine the 18 Mortality of the health issue using the of the health issue using the mortality rate of the health data from the research study data from the research study issue using the data from the and performing the appropriate and performing calculations, research study calculations but calculations are inappropriate, or determination has gaps in detail, clarity, or logic Health Issue: Odds Interprets the odds ratio to Interprets the odds ratio to Does not interpret the odds 18 Ratio determine the strength of determine the strength of ratio to determine the strength association between the association between the of association between the potential sources of exposure potential sources of exposure potential sources of exposure and the disease and the disease, but response and the di sease has gaps in detail, clarity, or accuracy Articulation of Submission has no major errors Submission has major errors Submission has critical errors 10 Response related to citations, grammar, related to citations, grammar, related to ci tations, grammar, spelling, syntax, or organization spelling, syntax, or organization spelling, syntax, or organization that negatively impact that prevent understanding of readability and articulation of ideas main ideas Total 100% Hello, I have 2 different assignments that are due on Sunday 12.02.18: MILESTONE 1 and MILESTONE 2.Please note that these are TWO different assignments that coincide together, therefore TWO different IHP 330 Milestone Two Guidelines and Rubric Overview: For your final project, you will complete an epidemiological research study report based on the provided research study, “ Salmonella in the Caribbean .” Throughout the course, you will complete milestone assignments that are drafts of specific sections of the final project. Because these milestone assignments are drafts of the final project, you will notice that some critical elements in the rubric are identical to those in the Final Project Rubric. In your first Milestone, you reviewed the research study and analyzed the health issue at the core of the research study. In this second Milestone, you will summarize key elements of the research study and analyze the population impacted by the health issue. Additionally, you will complete Part F of the Health Issue section, in which you will draw connections between the population and public health issue. Prompt: To begi n, review the final project research study, “Salmonella in the Caribbean.” Then, address the following critical elements in your short paper: I. Population : In this section of your final project, you will summarize key elements of the research study and anal yze the population impacted by the health issue. A. Summarize the people, places, and times represented in the research study to provide context for your report. B. Describe three social and behavioral determinants strongly associated with the public health issu e in the research study. Be sure to cite specific examples from the research study to support your description. C. Describe three known disparities associated with the health of the specific population. Be sure to cite specific examples from the research stud y to support your description. D. Explain how the identified social and behavioral determinants relate to the disparities you identified, and explain your reasoning. II. Health Issue : F. Using your research and analysis, draw connections between the population and public health issue. Consider where you identified common elements, what you learned about the public health issue by studying the population, and what you learned about the populatio n by studying the public health issue. Rubric Guidelines for Submission: Your paper should be a 1 - to 2 -page Microsoft Word document, with double spacing, 12 -point Times New Roman font, one - inch margins, and APA formatting and citations. Critical Elements Proficient (100%) Needs Improvement (7 5%) Not Evident (0%) Value Population: Summarize Summarizes the people, places, and times represented in the research study to provide context for the report Summarizes the people, places, and times represented in the research study to provide context for the report, but response has gaps in detail, clarity, accuracy, or support Does not summarize the people, places, and times represented in the research study to provide context for the report 18 Population: Social and Behavioral Determinants Describes three social and behavioral determinants strongly associated with the public health issue in the research study Describes three social and behavioral determinants strongly associated with the public health issue in the research study, but response is missing one or more determinants or has gaps in detail, clarity, accuracy, or support Does not describe three social and behavioral determinants strongly associated with the public health issue in the research study 18 Population: Disparities Describes three known disparities associated with the health of the specific population Describes three known disparities associated with the health of the specific population, but response is missing one or more disparities or has gaps in detail, clarity, accuracy, or support Does not describe three known disparities associated with the health of the specific population 18 Population: Determinants Relate to the Disparities Explains how the social and behavioral determinants relate to the disparities identified with reasoning Explains how the social and behavioral determinants relate to the disparities identified with reasoning, but response has gaps in detail, clarity, accuracy, or support Does not explain how the social and behavioral determinants relate to the disparities identified with reasoning 18 Health Issue: Connections Draws connections between the population and public health issue Draws connections between the population and public health issue, but response has gaps in detail, clarity, accuracy, or support Does not draw connections between the population and public health issue 18 Articulation of Response Submission has no major errors related to citations, grammar, spelling, syntax, or organization Submission has major errors related to citations, grammar, spelling, syntax, or organization that negatively impact readability and articulation of main ideas Submission has critical errors related to citations, grammar, spelling, syntax, or organization that prevent understanding of ideas 10 100% Total
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